|Main Features: |
Includes routines for:
· Examining intensity versus
time for individual pixels or spatial averages of pixels.
· Examining intensity versus time for all pixels.
· Many filtering and comparison options are available for both
of the above.
· Images of frame subtractions to provide maps of the signals.
Movies to display signal propagation.
NeuroPlex's first user interface (shown
at left, Enlarge) for analysis contains two windows for viewing
data. The right window, called the Page Display, displays
data from all pixels as they are positioned in the array.
The Trace Display (left window) provides a larger view of
data from a reduced number of pixels or electrode inputs.
Pixels can be selected for this more detailed examination
by pointing and clicking on the traces in the Page Display.
A variety of low- and high-pass filters and display adjustments
are available for each display.
The Trace Display also provides additional controls for averaging
over diodes, superimposing and scaling traces.
second analysis interface within NeuroPlex provides pseudocolor
displays of the data (shown at right, Enlarge). Options for
pseudocolor image displays include single frame, multiple
frame, and movie displays of the time series. For anatomical
reference, the pseudocolor images can be overlaid on an image
of the preparation. A variety of color schemes, scaling, and
filtering options are available.
Two different kinds of data display
are provided. The first is intensity vs. time for the individual
detectors and the second is a simultaneous pseudocolor display
of intensity for all detectors at a particular time. A long
series of such pseudocolor frames can be played as a movie.
Intensity VS. Time:
There are two display windows on the
main NeuroPlex widget. The Page Display displays intensity
versus time for all detectors (plus 8 BNC channels) in the
arrangement of the photodiode array. The individual traces
can be displayed with an enlargement on the Trace Display
by clicking them individually on the Page Display. The two
displays can be filtered and processed separately. In addition
to the usual X and Y gain adjustments, the following kinds
of high- and low- pass filtering are available:
· and binomial.
Traces can be displayed as intensity change
( I) vs. time or as fractional intensity change ( I/I) vs.
The Page Display can be modified by a fudge
array (replaces bad detectors with the average of the four
adjacent detectors) or an omit array (replaces detectors with
no signal with a flat line).
The Trace Display can display:
1. The spatial averages of selected
2. The FFT of selected traces,
3. The correlation between traces,
4. Spaced or superimposed traces,
5. Superimposed traces can be scaled to equal height for comparing
time courses, and
6. Both the Page display and the Trace display can be exported
as a TIFF file, a PostScript file or an ASCII file.
The filtered data can be made into
pseudocolor movie frames and then be viewed as individual
frames, or multiple frames in one window, or as an animated
movie. The color of the frames can be manipulated by using
different color tables and four different scaling schemes.
The scaling schemes are:
· Variable (each detector will have
the maximum and the minimum color at some time in the trial),
· Fixed; using the trace with the largest signal,
· Fixed; using a user chosen detector, and
(When you've tried all these options, it will be clear why
it is called pseudo-color).
The movie frames can also be overlayed
onto a high resolution CCD image of the preparation by importing
its TIFF or BMP file. The movie can be saved as a movie file
and the file can then be read and replayed by NeuroPlex. The
display on the movie window can be exported as a TIFF