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Description: Description: NeuroPlex-II Photodiode Array Imaging System
Installation & Testing Procedures For NeuroPDA-III

 

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SOFTWARE

 

 

 

 

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  NeuroPlex

  Turbo-SM

 

 

 

 

 


 

 

 

 

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HARDWARE

  FastCMOS-128X

 

  NeuroCMOS-DW

 

 

 

 

 

  NeuroCCD-SM/SMQ

 

 

 

 

 

  NeuroCCD-SM256

 

 

 

 

 

 

 

 

 

 

 

  NeuroPDA-III

Description: Description: Description: Description: Description: Description: Description: Description: Description: Description: Description: http://www.redshirtimaging.com/support/images/icon_reddot.gif Installation & testing

Description: Description: Description: Description: Description: Description: Description: Description: Description: Description: Description: http://www.redshirtimaging.com/support/images/icon_reddot.gif Hardware Limitation

 

 

 

 

 

 

  MacroScope-II & -IIa

 

 

 

 

 

  Shutter

 

 

 

 

 

 

 

 

 

 

 

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INFORMATION

 

 

 

 

 

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Description: Description: Propagating population signal from the turtle visual cortex. Provided by Jim Prechtl, Larry Cohen and David Kleinfeld, Yale University and UCSD.

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Before installing the system, please read the important precautions listed on the Hardware Limitations web page.

 

Unpacking

                                              

1.      Check for dents or damage to all packing boxes. If damaged, please make a record.

2.      Unpack all components. You should find the following:

o    Diode  array

o    Power supply

o    Interface panel

o    Computer


Description: Description: C:\MyRSI\website\web 2010\site_build\support\images\icon_redarrow.gifSCHEMATIC DIAGRAM of the NeuroPDA-III System
     (thumbnail shown at right) 

 

Description: Description: NeuroPlex-III Schematic Diagram: Click to Enlarge

Checking the Power Supply Unit

a)    Line voltage. Make sure your line voltage (110V for US and Japan, 220V for European users) matches the label on the power supply unit.  If not, do not turn on the power supply. Contact immediately WuTech (wuj@georgetown.edu) or your dealer.

b)   Output voltage. Connect the power supply to the wall  plug and the power supply "chassis" to the main ground in your laboratory. Turn on the power supply (the array should NOT be connected to the power supply at this point). The two indicator lights on the power supply should be on. Use a voltmeter to measure the output voltages  of the power supply. These should be +15V (red to green) and -14.2V (black to green).

c)    Short circuit protection.  Turn the power supply off and use a piece of wire to short the +15v to the ground connector. Now turn on the power supply. The indicator light for the +15V should not  turn on but the light for the -14.2V should light normally. Remove the wire - the +15V light should turn on, indicating the over current protection is working properly. Repeat the test for the -14.2V supply (by shorting the -14.2V to ground). If  the indicator lights do not turn back on after the shorting wire is removed, a fuse (inside the power supply) may have blown. Contact WuTech or your dealer.


Checking the diode array


a)    Mount the diode array on microscope.
  Before mounting remove the protection cover from the array aperture. The diode array can be mounted on the image port of a microscope/macroscope via a "C" mount. If the image port is not mechanically strong, or if the array is mounted horizontally,  add mechanical support for the array. (See also Mounting the NeuroPDA-III on a microscope.)   If  you opt not to mount the array at this point, lay it on a table with the connector side up and uncover the aperture.

b)   Connect the array to the power supply.
Make sure the power supply unit is OFF. Identify the speaker wires leading out of the array, labeled "Power Cable" and "Ground Cable" and connect them to the power supply unit: The red connector (of the red speaker wire) goes to the +15V connector on the power supply; The black connector (marked with a black tape, on the uncolored speaker wire) goes to the -14V. The Ground Cable (speaker wire with green or blue connector) connects to the ground connector on the power supply unit. Do not turn on the power supply yet. Description: Description: Switch positions for PDA-III

c)   Connect the array control cable to the control panel. Connect "Cable A" (a 40 lead flat cable) from the array and "Cable A'" leading out of the control panel to each other. Set the "Array Control" switches on the panel to: 10Mohm, X1 (See figure 2).

d)   Power on the array.
Turn on the power while watching the +/-15V indicator lights on the power unit. These should light normally. The green light on the Array Control section of the panel should also light. If any of these lights do not turn on or look dim, turn off the power supply and contact Wutech or your dealer.
Never power the array without connecting it to the Array Control.
Always set the array gains to 10Mohm,X1 when turning the power on/off (See figure 2).

e)    Corner lights on the array
. Turn on the "corner light" switch on the control panel and look at the array aperture. The fiber optics aperture is surrounded by red and green light guides. These lights are there to help orient the optical detector map. The orientation of the array is shown in Figure 4.

f)    Testing the diode array. Connect one of the "Optical Output" channels (located on the center section of the control panel) to an oscilloscope (vertical gain = .5V/div). While the array power is on, set the array controls to 10M, X100.  Provide a light signal (see below) to the array aperture and watch the output signal on the oscilloscope: the voltage change should correlate with the light intensity modulation.
If the array is mounted on a microscope, use the microscope light as the signal: Adjust the light level to be comfortable to the eye and approximately eve across the field of view. Switch the light from the eyepiece to the array. Use a piece of rigid paper (like an index card) to temporarily block the light (wave the card through the light path below the condenser). If you don't see a signal,  readjust the light so it is not too bright to the eye, then switch back the light from the eyepiece to the array . If the array is not mounted on the microscope, use the room lights to check it. Room light will usually provide a 120 Hz  fluctuation. Covering/uncovering the array aperture should change the amplitude of this modulation as seen on the oscilloscope. Different combinations of gains provide different sensitivity to the light. The two switches on the left of the "array control" panel (Figure 2) determine the the first and second stage gains. Changing the gain switch from x1 to x100 results in a 100 times larger output signal (2nd stage). Changing the first stage gain (I / V switch) from 10M to 100M, the array sensitivity grows 10 times. Note that at normal room light, using 100M for the first stage may saturate the array (flat line, no signal). This is normal and will not damage the array.
After testing the array, turn off the array power and proceed to the next stage.



Checking the computer and software.   


a)    Connecting the control panel to the computer. Turn
off the computer. Identify Cables B and C from the control panel (Figure 1) and connect them to the MicroStar Card. Connect the MicroStar card to the computer via the MicroStar Cable (Figure 1). At this time do not connect other cables from the control panel and leave the array power off. Now boot up the computer and watch the power indicator lights go on the control panel (Figure 2). All three lights for the computer power should be on, indicating that Cable B and C are connected correctly and the panel is powered. If none of the lights are on, check the connection of the MicroStar cable (Figure 1). If any of the lights is off, turn off the computer and check the connections of the cables, then try again. If any of the indicators fail to lit contact WuTech.Description: Description: PDA-III Digital output panel
Identify Cable D of the control panel and connect it to the "Digi-out" port  on the back of the computer. Cable D connector is a female 25 pin "D" connector, similar to the 2nd serial port on some computers. The "Digi-out" port is usually clearly marked. Alternatively it is located next to the MicroStar A/D convertor connector. If Cable D is correctly connected and the computer is on the green indicator light on the digital output section of the control panel (Figure 3) should lit.

b)   Testing the digital output. Run the Neuroplex software and take data (See below). The lights on each Digital output channel should blink indicating execution of the control command. Briefly, the "lamp" and "shutter" lights will be on for the duration of acquisition; "reset" will blink once at the beginning of a run and "stim" will blink when the stimulation command is issued (Figure 3). The time and duration of the "reset" command can be adjusted with the software, as discussed in the software manual. The expected blinking indicates the output section is working properly.

Description: Description: C:\MyRSI\website\web 2010\site_build\support\images\PDAIII-ApertureOrientation.jpgc)   Testing the analog input. There are 8 analog inputs channels in the H469-V allowing for simultaneous recording of eight different electrical signals  (electrodes, EEG, EKG, respiration, etc.) during imaging. The input channel connectors are located on the control panel. In the software they are labeled 465-472. To test these channels feed a 1 volt p-p, 40 Hz sine wave into one of the channels (e.g. #465). Run Neuroplex, taking  at least 1024 points per sweep. Display channel 465 on the left (trace) panel of Neuroplex. Repeat this for all other analog channels.

 

Testing the whole system.

This is the last and most important step of the installation. Before this step, both the array and the computer/control panel should be connected and properly tested (see above). Do not carry out the whole system test if you found problems during the previous tests.

 a)    Connecting the array to the control panel. Turn off the power to both the computer and the diode array. Identify Cable A from the array and mate it  with Cable A' from the control panel (see Figure 1). Connect the "Chassis Cable" from the array to the connector on the computer chassis.

b)    Power on the whole system.
Always remember to turn on the computer before turning on the array. The power supply indicators on the control panel (Figure 2) are designed to help the user remember the "power on" sequence: You can have the computer on without the array powered up, but never allow a situation where the array is powered and the computer is off. Also, before turning on the array power, set the array gain to 10M, X1 (Figure 2).


c)   Testing the system. Run Neuroplex (see Running Neuroplex below) and acquire data.

d)   Dark Noise. Measure the dark noise using the fastest acquisition rate. In addition to determining the size of the dark noise by measuring its RMS value, determine its frequency content by using FFT option under Trace. There should be little or no line frequency (or harmonics) noise as well as no increase at high frequencies (which would indicate oscillations). A flat spectrum is good.

e)   Bad channels.  Illuminate the diodes approximately evenly. Take data and examine the Page Display screen, looking for diodes which don't respond or are excessively noisy. Typical system has 5 or fewer bad channels. If you find more than five it is possible that the cards in the box are no longer properly seated. Very carefully remove the top of the diode array and check to see that no card has shifted. Carefully replace the top of the diode array. (You can use either the Fudge Array or Omit Array to replace the bad detectors with data from surrounding detectors or with flat lines).

This is the final step of the installation. Important hardware limitations can be found in Support Issues: NeuroPDA-III Hardware Limitations.


Running Neuroplex:

To take data use the following procedure:

Click on the NeuroPlex icon on the desktop to run the program. When the NeuroPlex window is opened, use Setup >> New Page and Trace Screen Size to make the Page and Trace screens have the desired size. Do Acquire >> Photodiode Array >> Number of Points so that the trace duration is about 1 sec. Then Go >> OK >> Start to take data.


Mounting the NeuroPDA-III on a microscope.

With a proper adapter, the NeuroPDA-III can be mounted on a C-mount port, TV port or any other port with real image. However, we recommend that it will be mounted on a "simple" port for maximum light throughput. Most research microscopes provide this kind of high throughput port. In general, the fewer lenses between the objective and the fiber optics, the higher the throughput.
On the housing, outside the optical aperture, there are three mounting holes tapped with 10-32 thread. These three holes fasten three 10-32 screws and a plate (red color) with a C-mount thread on the aperture. This C-mount thread can be used to interface with most microscopes. Spaces can be added between the array body and the C-mount plate, in order to obtain parfocality between the array and the ocular of the microscope.

 

 

 

Congratulations and enjoy the new system.

Jiang-young Wu, Ph.D.
WuTech Instruments
2 Oceania Court
Gaithersburg, MD 20877
U.S.A.

Chun Falk, Ph.D.
Lawrence Cohen, Ph.D.
RedShirtImaging, LLC
2 Stoneleigh Road
Fairfield, CT 06825
U.S.A.