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Description: Description: Description: Description: NeuroCCD-SM256 Cooled-CCD Imaging System
Installation

 

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  SOFTWARE

 

 

 

 

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  NeuroPlex

  Turbo-SM

 

 

 

 

 


 

 

 

 

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  HARDWARE

 

 

 

 

  FastCMOS-128X

 

  NeuroCMOS-DW

 

 

 

 

 

  NeuroCCD-SM/SMQ

 

 

 

 

 

  NeuroCCD-SM256

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Description: Description: Description: Description: Description: Description: Description: Description: Description: http://www.redshirtimaging.com/support/images/icon_reddot.gif Hardware Limitation

 

 

 

 

 

 

  NeuroPDA-III

 

 

 

 

 

 

 

 

 

 

 

  MacroScope-II & -IIa

 

 

 

 

 

  Shutter

 

 

 

 

 

 

 

 

 

 

 

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INFORMATION

 

 

 

 

 

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Description: Description: Description: Description: Backpropagation of action potential in neocortical pyramidal neuron. Measured using NeuroCCD, frame interval .37ms. Provided by Srdjan Antic and Dejan Zecevic, Yale University.

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  1. Connect cables for the system following the instructions on the Schematic Diagram for the NeuroCCD-SM256 shown below. 
  2. Connect "Stim" on the Control Box (see below) to your stimulator input trigger.
  3. Connect "Shutter" on the Control Box to your shutter driver.
  4. Connect the "monitor" signal on your stimulator to Input 1 on the Control Box.



Components:

1.

Camera Head.

 

 

A high-speed/high spatial resolution back-illuminated CCD camera which acquires continuous frames at a speed up to 100Hz at 256x256, 125Hz at 128x128, 250Hz at 84x84, and 300Hz at 64x64.

 

2.

 

Camera Controller

 

 

It communicates with the camera and the EDT card inside the computer.

3.

 

Optical Coupler  

 

 

Resizes the image created at the microscope image plane onto the 6.66mm2 CCD chip at a ratio of 3:1. C-mount is provided for standard microscope mounting.

4.

 

Power Supply

 

 

Power supply for the camera.

5.

 

Computer

 

 

The data acquisition unit with an EDT card that communicates with the camera via the controller and a Microstar A-to-D/D-to-A board that simultaneously records up to 8 channels of electrical data and controls the experiment.

6.

 

Control Panel

 

 

The control panel is the central plug-in point for various signals.

1. It sends the trigger signal to the camera via line c.
2. It sends out the control signals from the D-to-A to control the shutter (line i) and the stimulator (line j).
3. It receives electrical signals via the top 8 channels ( ).
4. It sends analogue output signals via the g BNCs.

 

Connections:

     a - Power supply to camera controller.

b - Cable connecting the camera head and the controller.

c - Clock trigger line from the control panel to camera controller. **

d - Camera controller to EDT board.

     e - Cable connecting Microstar A-to-D/D-to-A board to the control panel.

f - 8 BNC inputs.

g - Analog outputs (See Software Manual).

h - External Trigger.

     i - TTL output that controls the shutter during data acquisition.

     j - TTL output that triggers stimulator at the beginning of acquisition.

 

 ** If you have two cameras and are only running one, turn the 2nd camera on or disconnect it from this line.

 

Description: Description: Description: Description: C:\MyRSI\website\web 2010\site_build\support\images\icon_redarrow.gifControl Box

This unit is the external connection to the A-to-D board (DAP820/DAP840) and thus has the capacity of sending out TTL and other analog signals as well as accepting incoming signals.

Below is an image describing the inputs/outputs of this box. An equivalent image is glued to the top of every control box shipped by RedShirtImaging.

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Inputs 1-8 :

These 8 BNCs are intended for input of electrical data (Voltage, Current etc..) to be recorded in synchrony with the optical data. Connect the output from your amplifier(s) to any of those inputs. The inputs are digitized at 12 bit accuracy.

 

Clock Trigger:

The output from this BNC is a trigger to the camera for each frame acquired. This trigger is essential to the data acquisition and should be connected to the camera controller. (see diagram ).

 

Shutter:

Connect this BNC to the "Pulse Input" BNC on your Shutter Control Box and set the later to "remote control" (Names relate to the Uniblitz shutter control and might vary). The data acquisition module of NeuroPlex controls the shutter via this output.

 

Stim:

Connect this BNC to the "External trigger" of your stimulator. Any delay desired between the start of recording and the stimulus should then be set on your stimulator.

 

Analog Output:

Any signal sent by the NeuroPlex data acquisition function "Analog Output", will be output through this BNC. Thus - in order to send a voltage command or a waveform - this BNC should be connected to the appropriate external input on your amplifier.

 

External Trigger:

The purpose of this BNC is to synchronize the data taking with an external event (e.g. heartbeat, sniffing, EKG). When using the "external mode" (see software manual), after the "Start" button has been pushed, the program looks for an input voltage (2.5V or more) at this BNC to trigger the acquisition. The width of a repetitive triggering pulse should be shorter than the acceptable jitter in the relationship between the recorded event and the trigger. If the trigger signal is long (e.g. a duty cycle of 50%) different trials will be triggered at different phases of the cycle.