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Description: Propagating population signal from the turtle visual cortex. Provided by Jim Prechtl, Larry Cohen and David Kleinfeld, Yale University and UCSD.

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Turbo-SM
(An acquisition software for Neuro/CardioCCD and Neuro/CardioCMOS systems)

Software Instalation

We install Turbo-SM on the computer shipped to you with your new system.  The computer has been loaded with the drivers for the camera. If you computer crashed and you need to reinstall everything from scratch, please call us.



How to use Turbo-SM :


1.   The camera needs to be turned on before starting the software. If the camera was turned off and on while the software is open, click Reset Camera to reclaim the device pointer.
2.The camera acquisition setting is under Change Settings where you can select configuration (for base frame rate and on-chip binning), exposure time (or frame interval), number of frames (for Recording), and the experiment name (folder name under Turbo-SM\SMDATA\. The file names will be the same as the folder name with increments.
3.Front panel :
a).     Live – Live images from the camera, used for positioning and focusing on the preparation, adjusting gain or light level (see 7 for tips). The Shutter-LED bit goes up when it’s live and goes back down when it stops.
b). Record – Data acquisition for predefined number of frames. The data will be written to the file as defined. If a built-in A/D card is included in the camera, the BNC data will be saved as a binary file under the same name with extension tbn. The recording can also be triggered with an external trigger source connected to External Trigger. A pre-trigger delay in milli-seconds can also be set.
The Shutter-LED bit goes up during recording. The TTL out bit goes up briefly at the beginning and can be used as a Stimulus signal.
c).Single Frame – A quick view of one image with shutter opening and closing.
d).Dark Frame - Acquire a Dark Frame.  You can choose to use it to subtract from the Live or Single Frame display. Since the camera has multi-channel readout, Dark Frame Subtraction will get rid of the slight discrepancy in amplifier offsets of each channel. The dark frame will be automatically be saved at the end of the file when recording if it exists (indication: Dark Subtracted not grayed out).  If you forgot to take a dark frame before recording, you can take one and select File>>Resave Dark Frame. Be aware that Set Dark Offset can change the dark frame, and you need to take a new dark frame when changing configurations or gains.
e). Set Dark Offset only needs to be used when there is a significant difference between different channels in dark level. Light should be off or the shutter closed when using this function.
4. Image Display: You can select ROIs on the image to view waveforms, 3 traces maximum (last 3 selected) at one time. There is a toolbox under the histogram. It allows you to choose different modes of ROI selection (kernel, box, or draw irregular shape). The waveform for BNCs can be selected by selecting BNC 1,2,3… There are also buttons for Play/Pause movie and Next and Previous frame, and a slider for image gray-level scaling. Auto Scale resets this slider.
5. Trace Display: There are 5 sliders that control Y-gain, high pass and low pass filtering of the trace, as well as X-expansion and the shift of beginning point.
6. Under File, the image file can be saved as FITS, which can be read in ImageJ. Neuroplex will also read the files created by Turbo-SM.
7.Tips:  To optimize S/N ratio, select the frame rate (configuration) and then start with the lowest Camera Gain (1X) and use the brightest light for your experiment without saturating the camera or causing problems to the preparation. The brightest light will give the highest signal to noise ratio. Once you are set to the brightest light level and if the light level is less than 1/3 of saturation, increase Camera Gain to the highest possible setting without saturation. The higher gain, the lower the read noise.
8.Windows 7 system and display settings: If your computer came with the camera, there is no need to change the settings. However, if you noticed performance issues, see file Windows7 System Changes For Running Turbo-SM.txt in C:\Turbo-SM\ for reference.



    


  
   
  
    
  







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