RedShirtImaging, LLC. MacroScope: Dual Ported 2X and 4X Microscope
Installation

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SOFTWARE

 

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  NeuroPlex

  Turbo-SM

 

 


 

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HARDWARE

 

  FastCMOS-128X

 

  NeuroCMOS-DW

 

 

  NeuroCCD-SM/SMQ

 

 

  NeuroCCD-SM256

 

 

  NeuroPDA-III

 

 

 

 

 

  MacroScope-II & -IIa

 

 

  Shutter

 

 

 

 

 

  Macroscope

 

 

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INFORMATION

 

 

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Backpropagation of action potential in neocortical pyramidal neuron. Measured using NeuroCCD, frame interval .37ms. Provided by Srdjan Antic and Dejan Zecevic, Yale University.

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WARNING
While most of the MacroScope parts are robust, two connections are made with "instant" or "crazy" glue and are relatively weak. These are the connection of the lens to the metal block just above it and the connection of the dichroic mirror to its metal support. Care in handling is in order. In case of accidents, repairs can be made with instant glue for the lens and 5-minute epoxy for the dichroic mirror.
Illumination
To provide even illumination of a large field use a lamp with a large filament. This is especially important for the 2X lens. A 24 volt, 300 watt, tungsten-filament lamp has a relatively large filament.
 
Mounting
While the photograph of the MacroScope on the web site shows the MacroScope to be free standing (separate from a camera), we find it more convenient to attach the MacroScope directly to the camera via the vertical C-mount. This fixes the alignment and the distance between the lens and camera. RedShirtImaging provides mounting hardware for NeuroPDA or NeuroCCD cameras with the MacroScope.
Optical Alignment
The MacroScope is designed to form a real inverted image 1.7 cm above (past) the end of either C-mount. For both the 2X and the 4X objectives a working distance of about 1.2 cm should form an image at the correct location. 
 
When shipped, the dichroic mirror was aligned so that it illuminated the preparation evenly and it was centered. You should check this alignment. First, aim the light source so that it is perpendicular to the imaging path and focus an image of the filament or arc at the back of the lens (Kohler illumination). Take a piece of graph paper and position it just below the lens with your Z-axis positioner and draw an outline of the bottom of the lens. Then mark the center of the circle with a black spot. Lower the graph paper by 1.2 cm. A centered image should be formed 1.7 cm past the end of the C-mount. In addition, the illumination should be correctly centered on the graph paper and should be reasonably even. You can adjust the centering using the three thumb screws on the back of the dichroic mirror holder and by rotating the dichroic holder (after loosening the two set screws). 
 
When shipped, the beam diverter for the auxiliary C-mount was aligned so that it projected a centered image onto an auxiliary CCD camera. You will probably need to adjust this alignment with the three thumb screws on the back of the diverter.

Changing Lenses
The Linos parts fit together rather tightly. In order to change lenses you will probably need to loosen all 12 set screws that are used to attach the lens holder, the mounting plate, and the cube to the four stainless steel bars that are used to connect the three parts.
Additional Parts
Many of the parts in the MacroScope are obtained from Linos Photonics, Inc., Milford, MA 01757 USA. If you wish to make modifications to the MacroScope, we are happy to give advice or you can consult their catalogue. We can provide a parts list.