Description: RedShirtImaging, LLC.

Description: NeuroCCD Cooled-CCD Activity Imaging System
Installation

 

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SOFTWARE

 

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  NeuroPlex

  Turbo-SM

  

 

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HARDWARE

 

  FastCMOS-128X

 

  NeuroCMOS-DW

 

 

  NeuroCCD-SM/SMQ

 

 

  NeuroCCD-SM256

 

 

  NeuroPDA-III

 

 

 

 

 

  MacroScope-II & -IIa

 

 

  Shutter

 

 

 

 

 

  NeuroCCD

 

 

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Description: Description: Description: Description: Description: Description: Description: Description: Description: Description: http://www.redshirtimaging.com/support/images/icon_reddot.gif Start-Up Procedure

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Ask The Expert

 

 

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INFORMATION

 

 

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Contact Us

 

 

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Users

 

 

 

 

 

DISTRIBUTORS

 

 

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Australia, New Zealand

 

 

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British Isles, Benelux, France, Italy, Scandinavia, and Spain

 

 

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China, Hong Kong

 

 

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Japan

 

 

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Switzerland

 

 

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Taiwan

 

 

 

 

 

 

 

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Description: Backpropagation of action potential in neocortical pyramidal neuron. Measured using NeuroCCD, frame interval .37ms. Provided by Srdjan Antic and Dejan Zecevic, Yale University.

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  1. Connect cables for the system following the instructions on the Schematic Diagram for the NeuroCCD shown below.
  2. Attach the IDL dongle onto the printer port.

Description: C:\MyRSI\website\web 2010\site_build\support\images\icon_redarrow.gifSchematic Diagram of the NeuroCCD System
Description: NeuroCCD Diagram

Units

Description: 1.

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FastOne Camera

 

 

FastOne is a high-speed back-illuminated CCD camera which acquires continuous frames at a speed up to 2.7 Kfps.

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Description: 2.

 

Optical Coupler

 

 

Resizes the image created on the microscope image plane onto the 1.44 mm2 CCD chip at a ratio of 10:1. C-mounts are used for standard microscope mounting.

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Description: 3.

 

Fan Controller

 

 

The fan controller is an electrical on/off switch that turns off the fan in response to a binary pulse sent out by the computer during the data acquisition. It is important, in most cases, to turn the fan off because the fan introduces vibration noise which becomes prominent in the image field where there is spatial contrast.

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Description: 4.

 

Power Supply

 

 

Power supply for the camera.

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Description: 5.

 

Computer

 

 

The data acquisition unit with: 1. a PCI card that connects to the A-to-D inside the camera and 2. a second A-to-D/D-to-A board that records up to 7 channels of electrical data and controls the experiment.

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Description: 6.

 

Control Panel

 

 

The control panel is the switch board for various signals.

(1). It receives the trigger signal from the camera via line h.
(2). It sends out the control signals from the D-to-A to control the fan (line i), the shutter (line j), the stimulator (line k) and the synchronizing LED (line l).
(3). It receives electrical signals via the top 7 channels (Description: C:\MyRSI\website\web 2010\site_build\support\images\channel_7.gif ). The 8th channel (Description: C:\MyRSI\website\web 2010\site_build\support\images\channel_8.gif ) is currently used for synchronizing signals that synchronize the optical data and the electrical data. Do not use this channel.

Connections:

a. Connects the power supply to the camera.

b. Control line between the camera and its PCI board. (b-f are fiber optic cables)

c. Connection between the camera and the PCI board -- Channel 0.

d. Connection between the camera and the PCI board -- Channel 1.

e. Connection between the camera and the PCI board -- Channel 2.

f. Connection between the camera and the PCI board -- Channel 3.

g. Connects the 2nd A-to-D to the control panel. The BNC connectors on the front face of the control panel are connected to the inputs and the outputs of the A-to-D.

h. A trigger line from the camera to the control panel. This triggers the electrical data acquisition and the experimental control of the 2nd A-to-D. The connector on the camera is labeled as "Shutter Sync".

i. Fan control line that turns the camera fan off before the data acquisition to eliminate the vibrational noise caused by the fan.

j.  Shutter control that opens the shutter during data acquisition.

k. Trigger pulse for a stimulator.

l. This output provides synchronization signals to the LED and BNC8 (BNC8 is connected internally). Because the first triggering pulse from the camera does not correspond to the first frame acquired, it is important to have this synchronization signal. The pulses marked by a dashed line in the diagram below will appear on the 8th electrical trace and in the optical data when the user elect to include the synchronization test.

Diagram of Output Signals from i-l
Description: Diagram of Output Signals from i to l