Optical Coupler (10:1)
The coupler has C-mount connections on each end. The end with the small diameter lens is screwed into the FastOne camera. The other end is screwed to a C-mount port on the microscope. The coupler has two adjustments, one for focus and the second for centering.
The focus adjustment can be used to make the camera and the microscope eyepieces parfocal.
1. Focus on an object using the eyepieces.
2. Loosen the connection between the coupler and the camera.
3. Loosen the two Allen screws on the part of the coupler closest to the camera.
4. Using the Acquire Continuously function in NeuroPlex, rotate the part of the coupler closest to the camera until the focus is sharpest. (Items 1-8 of Start-Up Procedure)
5. Tighten the two Allen screws and the connection to the camera.
The centering adjustment can be used to make the camera and the microscope eyepieces have the same center.
1. Center an object using the eyepieces.
2. Loosen the three Allen screws on the part of the coupler distal to the focus adjustment.
3. Using the Acquire Continuously function, adjust the three Allen screws so that the object is centered. Then tighten the screws.
unit is the external connection to the A-to-D board (DAP820/DAP840) and
thus has the capacity of sending out TTL and other analog signals as
well as accepting incoming signals.
Inputs 1-8 :
These 8 BNCs are intended for input of electrical data (Voltage, Current etc..) to be recorded in synchrony with the optical data. Connect the output from your amplifier(s) to any of those inputs. The inputs are digitized at 12 bit accuracy.
Connect "Shutter Sync" on the camera to this BNC. This way - the camera triggers the electrical data acquisition by the AtoD board. (see diagram in NeuroCCD Installation).
Connect this BNC to the "Pulse Input" BNC on your Shutter Control Box and set the later to "remote control" (Names relate to the Uniblitz shutter control and might vary). The data acquisition module of NeuroPlex controls the shutter via this output.
Connect this BNC to the "External trigger" of your stimulator. Any delay desired between the start of recording and the stimulus should then be set on your stimulator.
Any signal sent by the NeuroPlex data acquisition function "Analog Output", will be output through this BNC. Thus - in order to send a voltage command or a waveform - this BNC should be connected to the appropriate external input on your amplifier.