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Description: NeuroCCD-SM Cooled-CCD Imaging System
Installation

 

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  SOFTWARE

 

 

 

 

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  NeuroPlex

  Turbo-SM

 

 

 

 

 


 

 

 

 

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  HARDWARE

 

 

 

 

 

  FastCMOS-128X

 

  NeuroCMOS-DW

 

 

 

 

 

  NeuroCCD-SMQ/SMQ-II

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  NeuroCCD-SM256

 

 

 

 

 

  NeuroPDA-III

 

 

 

 

 

 

 

 

 

 

 

  MacroScope-II & -IIa

 

 

 

 

 

  Shutter

 

 

 

 

 

 

 

 

 

 

 

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INFORMATION

 

 

 

 

 

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Description: Backpropagation of action potential in neocortical pyramidal neuron. Measured using NeuroCCD, frame interval .37ms. Provided by Srdjan Antic and Dejan Zecevic, Yale University.

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  1. Connect cables for the system following the instructions on the Schematic Diagram for the NeuroCCD-SMQ-II shown below.
  2. Connect "Stim" on the Control Box (see below) to your stimulator input trigger.
  3. Connect "Shutter" on the Control Box to your shutter driver.
  4. Connect the "monitor" signal on your stimulator to Input 1 on the Control Box.



Components:

1.

Camera Head.

 

 

A high-speed back-illuminated CCD camera which acquires continuous frames at a speed up to 2.0 KHz at 80x80, 3.0KHz at 40x40, and 5.0KHz at 26x26.

 

2.

 

Camera Controller

 

 

It communicates with the camera and the EDT card inside the computer. Also houses an AtoD/DtoA card to simultaneously records up to 4 channels of electrical data.

3.

 

Optical Coupler  

 

 

Resizes the image created at the microscope image plane onto the 1.92mm2 CCD chip at a ratio of 10:1. C-mount is provided for standard microscope mounting.

4.

 

Power Supply

 

 

Power supply for the camera.

5.

 

Computer

 

 

The data acquisition unit with an EDT card that communicates with the camera via the controller .

6.

 

Control Panel

 

 

The control panel is the central plug-in unit for various signals.

1. It sends out the control signals from the DtoA to control the shutter (line g) and the stimulator (line h).

2. It sends a general external trigger (line f).

2. It receives electrical signals via the top 4 channels ( ).

 

Connections:

     a - Power supply to camera controller.

b - Cable connecting the camera head and the controller.

c - Camera controller (AtoD) to control panel . 

d - Camera controller to EDT board.

e - 4 analog BNC inputs.

f - External Trigger.

     g - TTL output that controls the shutter or LED during data acquisition.

     h - short TTL output that triggers stimulator during acquisition.

 

 
 

Description: C:\MyRSI\website\web 2010\site_build\support\images\icon_redarrow.gifControl Box

This unit is the external connection to the AtoD board and thus has the capacity of sending out TTL and other analog signals as well as accepting incoming signals.

Below is an image describing the inputs/outputs of this box. 

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Inputs 1-4 :

These 4 BNCs are intended for input of electrical data (Voltage, Current etc..) to be recorded in synchrony with the optical data. Connect the output from your amplifier(s) to any of those inputs. The inputs are digitized at 12 bit accuracy.

 

Shutter:

Connect this BNC to the "Pulse Input" BNC on your Shutter Control Box and set the later to "remote control" (Names relate to the Uniblitz shutter control and might vary). The data acquisition module of NeuroPlex controls the shutter via this output.

 

Stim:

Connect this BNC to the "External trigger" of your stimulator. Any delay desired between the start of recording and the stimulus should then be set on your stimulator.

 

 External Trigger:

The purpose of this BNC is to synchronize the data taking with an external event (e.g. heartbeat, sniffing, EKG). When using the "external mode" (see software manual), after the "Start" button has been pushed, the program looks for an input voltage (2.5V or more) at this BNC to trigger the acquisition. The width of a repetitive triggering pulse should be shorter than the acceptable jitter in the relationship between the recorded event and the trigger. If the trigger signal is long (e.g. a duty cycle of 50%) different trials will be triggered at different phases of the cycle.